With the rise of antibiotic-resistant bacteria such as Methicillin Resistant Staphylococcus Aureus (MRSA), we must find alternatives to manufactured antibiotics. This is where my study on Manuka Honey comes in. General honey is well known for its anti-inflammatory and antimicrobial characteristics. Manuka honey claims to be more antimicrobial due to its higher MGO concentration. I’ll compare general honey and manuka honey and its ability to inhibit biofilm growth in Staphylococcus Aureus. Biofilms are polysaccharide films that encompass a colony of bacteria. This issue is because it acts as a barrier between the bacteria and the environment. There are also issues with treating biofilm-forming bacteria with antibiotics. I hypothesize that Manuka Honey inhibits biofilm growth in Staphylococcus Aureus better than General Honey.
On Tuesday, I plated E. Coli and tested the effectiveness of 4 samples to kill the bacteria. The sample included , Aloe Vera gel (store bought), Windex, Tap water, and 70% Isopropyl Alcohol. This experiment was pretty much used to keep me busy as I haven't yet been able to start a project. However when I came in Wednesday, I found some interesting results. The zone of inhibition was basically 0 for every sample, including the isopropyl alcohol. Although the tap water, being the control, and the Windex doesn't jump out as a surprise to me, the 70% Alcohol does. Originally I had two hypothesis's on why this occurred. One being that because E. Coli is a gut bacteria and therefore is built to withstand alcohol. The second theory suggest user error such as the letting the alcohol evaporate to much before I was able to put the disk on the plate. Because of the results on Wednesday and with the first hypothesis in mind, I chose to do another plate instead with Staphylococcus. I ch...
Comments
Post a Comment