Hi, everyone; this week has been quite busy in the lab. For the past few weeks, I've been repeating subculture due to not having enough time to do an experiment. This week, I finally had the time. I'll be completing a biofilm formation assay. It's extremely similar to what I did last year but with some tweaking. Outside of the lab, I've put my creative ideas to use for decorating for Halloween. I've also been putting some ideas to use for the robotics club and the Halloween ball. I'm super excited to see how everything will come out.
On Tuesday, I plated E. Coli and tested the effectiveness of 4 samples to kill the bacteria. The sample included , Aloe Vera gel (store bought), Windex, Tap water, and 70% Isopropyl Alcohol. This experiment was pretty much used to keep me busy as I haven't yet been able to start a project. However when I came in Wednesday, I found some interesting results. The zone of inhibition was basically 0 for every sample, including the isopropyl alcohol. Although the tap water, being the control, and the Windex doesn't jump out as a surprise to me, the 70% Alcohol does. Originally I had two hypothesis's on why this occurred. One being that because E. Coli is a gut bacteria and therefore is built to withstand alcohol. The second theory suggest user error such as the letting the alcohol evaporate to much before I was able to put the disk on the plate. Because of the results on Wednesday and with the first hypothesis in mind, I chose to do another plate instead with Staphylococcus. I ch...
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