This is the conclusion to my research.
"Unfortunately, due to the uncertainties of the data, no conclusion can be made. Due to time constraints, the research is unfinished. The only bacteria tested was Pseudomonas Aeruginosa. As well as no microdilution and testing of the efficacy of the honey were done. In the future, this research aims to work with P. Aeruginosa, S. Aureus, and S. Epidermis and incorporate the manuka honey. Physical examination shows a confirmed growth of a slimy substance in the test tube containing Pseudomonas Aeruginosa. In past research, evidence suggests that Manuka suggests that different concentrations inhibit the growth of the chosen bacteria."
Although my research was inconclusive, I don't feel bad. I can't wait to come back next semester and work out the kinks.
On Tuesday, I plated E. Coli and tested the effectiveness of 4 samples to kill the bacteria. The sample included , Aloe Vera gel (store bought), Windex, Tap water, and 70% Isopropyl Alcohol. This experiment was pretty much used to keep me busy as I haven't yet been able to start a project. However when I came in Wednesday, I found some interesting results. The zone of inhibition was basically 0 for every sample, including the isopropyl alcohol. Although the tap water, being the control, and the Windex doesn't jump out as a surprise to me, the 70% Alcohol does. Originally I had two hypothesis's on why this occurred. One being that because E. Coli is a gut bacteria and therefore is built to withstand alcohol. The second theory suggest user error such as the letting the alcohol evaporate to much before I was able to put the disk on the plate. Because of the results on Wednesday and with the first hypothesis in mind, I chose to do another plate instead with Staphylococcus. I ch...
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