Hi Everyone,
Another busy week. Mostly busy outside of school, and because of that, I had little time to work in the lab. I've been at a standstill for the last week, unsure what to do with my experiment. The Conga Red that we ordered, lost, and then had to re-order, didn't work as expected. I've dove a little deeper and found a more accurate but laborious option for detecting biofilms. On a better note, I've been able to really work on my research paper, which, in previous semesters, had typically been put on the back burner. Next week, I plan to dedicate my lab time to working on this new technique. Can you see the film in the tube?
On Tuesday, I plated E. Coli and tested the effectiveness of 4 samples to kill the bacteria. The sample included , Aloe Vera gel (store bought), Windex, Tap water, and 70% Isopropyl Alcohol. This experiment was pretty much used to keep me busy as I haven't yet been able to start a project. However when I came in Wednesday, I found some interesting results. The zone of inhibition was basically 0 for every sample, including the isopropyl alcohol. Although the tap water, being the control, and the Windex doesn't jump out as a surprise to me, the 70% Alcohol does. Originally I had two hypothesis's on why this occurred. One being that because E. Coli is a gut bacteria and therefore is built to withstand alcohol. The second theory suggest user error such as the letting the alcohol evaporate to much before I was able to put the disk on the plate. Because of the results on Wednesday and with the first hypothesis in mind, I chose to do another plate instead with Staphylococcus. I ch...
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