Hi Everyone,
Hope everyone had a nice spring break. I spent most of the break taking long naps and enjoying my birthday. I never realize how tired school and constantly being on the go makes me feel until it all comes to a stop. This week is about finding my groove again, but I also have to worry about the second exams for my science classes. When I returned from break, none of the bacteria turned black (or any color) on the conga red plates. Black would've indicated biofilm-forming bacteria. Josh and I are sure the pseudomonas aeruginosa is at least a biofilm-forming bacteria, which leads us to believe that something is wrong with the conga red in the agar plates. To prove our theory (pseudomonas aeruginosa being a biofilm-forming bacteria), we've put pieces of foil in TSB to promote biofilm growth. We are doing this because we noted that for biofilm to form, it needed a surface to adhere to.
On Tuesday, I plated E. Coli and tested the effectiveness of 4 samples to kill the bacteria. The sample included , Aloe Vera gel (store bought), Windex, Tap water, and 70% Isopropyl Alcohol. This experiment was pretty much used to keep me busy as I haven't yet been able to start a project. However when I came in Wednesday, I found some interesting results. The zone of inhibition was basically 0 for every sample, including the isopropyl alcohol. Although the tap water, being the control, and the Windex doesn't jump out as a surprise to me, the 70% Alcohol does. Originally I had two hypothesis's on why this occurred. One being that because E. Coli is a gut bacteria and therefore is built to withstand alcohol. The second theory suggest user error such as the letting the alcohol evaporate to much before I was able to put the disk on the plate. Because of the results on Wednesday and with the first hypothesis in mind, I chose to do another plate instead with Staphylococcus. I ch...
Comments
Post a Comment