Hi Everyone,
This week, I focused on my experiment's optical density part. Unfortunately, when I came in on Monday, I discovered the liquid had evaporated sometime over the weekend. I had to make a new sample, increasing the amount of media in each. On Thursday, I took the optical density of the new sample. It showed that all three types of honey inhibited growth. Manuka Honey 1 had the most potent MGO concentration and had the least growth. MGO, or methylglyoxal, is the compound accredited for honey's antimicrobial properties. The new sample showed evidence of cross contamination in the control well (media), so I'm testing the media to see if the whole bottle is contaminated. The next step is determining the minimum inhibitory concentration with a two-fold dilution.
On Tuesday, I plated E. Coli and tested the effectiveness of 4 samples to kill the bacteria. The sample included , Aloe Vera gel (store bought), Windex, Tap water, and 70% Isopropyl Alcohol. This experiment was pretty much used to keep me busy as I haven't yet been able to start a project. However when I came in Wednesday, I found some interesting results. The zone of inhibition was basically 0 for every sample, including the isopropyl alcohol. Although the tap water, being the control, and the Windex doesn't jump out as a surprise to me, the 70% Alcohol does. Originally I had two hypothesis's on why this occurred. One being that because E. Coli is a gut bacteria and therefore is built to withstand alcohol. The second theory suggest user error such as the letting the alcohol evaporate to much before I was able to put the disk on the plate. Because of the results on Wednesday and with the first hypothesis in mind, I chose to do another plate instead with Staphylococcus. I ch...
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