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Fall 2023: Week 7

Hi Everyone, This week, I focused on my experiment's optical density part. Unfortunately, when I came in on Monday, I discovered the liquid had evaporated sometime over the weekend. I had to make a new sample, increasing the amount of media in each. On Thursday, I took the optical density of the new sample. It showed that all three types of honey inhibited growth. Manuka Honey 1 had the most potent MGO concentration and had the least growth. MGO, or methylglyoxal, is the compound accredited for honey's antimicrobial properties. The new sample showed evidence of cross contamination in the control well (media), so I'm testing the media to see if the whole bottle is contaminated. The next step is determining the minimum inhibitory concentration with a two-fold dilution.

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