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Fall 2023: Week 6

(Lab's very own pink drink) Hi Everyone, I had a great week with my experiment. I am working on another process to measure the effect of Manuka honey on Staphylococcus aureus. I plan to use broth microdilution, typically used in microbiology, to determine a minimum inhibitory concentration. With this in mind, I'm hypothesizing that if Manuka honey inhibits the growth of S.A., then at some unknown concentration (discovered in the microdilution), the bacteria cell count will dramatically decrease. This week, I diluted each honey in 100ml of DI water to create a "stock solution." For a different test, I set up a microplate with wells containing variations of Muller Hilton Broth, S.A, and Honey's. I used a microplate photometer to measure absorbance. This is similar to the last test I did, but we're trying to eliminate some of the problems from that test.

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