Skip to main content

Fall 2023: Week 6

(Lab's very own pink drink) Hi Everyone, I had a great week with my experiment. I am working on another process to measure the effect of Manuka honey on Staphylococcus aureus. I plan to use broth microdilution, typically used in microbiology, to determine a minimum inhibitory concentration. With this in mind, I'm hypothesizing that if Manuka honey inhibits the growth of S.A., then at some unknown concentration (discovered in the microdilution), the bacteria cell count will dramatically decrease. This week, I diluted each honey in 100ml of DI water to create a "stock solution." For a different test, I set up a microplate with wells containing variations of Muller Hilton Broth, S.A, and Honey's. I used a microplate photometer to measure absorbance. This is similar to the last test I did, but we're trying to eliminate some of the problems from that test.

Comments

Post a Comment

Popular posts from this blog

Fall 2024- Week 5

Hi Everyone, This week was my first whole week in the lab. I focused mainly on calculations and locating everything I needed for the next few weeks. I also made fresh media this week. We made a few adjustments to last year's protocol in hopes that we get better jobs. We made adjustments by ordering flat bottom well plates to help bacterial biofilms stick better. We got an adhesive film to cover the plates to avoid too much evaporation and cross-contamination. By next week, I plan to plate some bacteria and measure the biofilms. (I had to autoclave my media and bottles to store it in)
Post a Comment
Read more

Fall 2024-Week 13

Hi everyone, I hope everyone had an ejoyable thanksgiving.There isn't much for me to say. I was able to finish my experiments before leaving for vacation last week. I plan on analyzing my results and finishing my research paper. I also plan to work on the final presentation. This week was also my last week for the organic chemistry lab and lecture. I'll be taking the organic chemistry final on monday so wish me luck.
Post a Comment
Read more

Fall-2024 Week 7

Staphylococcus aureus is considered one of the most prevalent pathogens in hospital-related infections. This strain of Staphylococcus are apart of the human microbiota, and they can contaminate medical instruments and implants and cause infections. (Chessa et al., 2016) Certain strains of Staphylococcus Aureus have gained the ability to resist the antibiotic methicillin. This strain is known as methicillin-resistant Staphylococcus Aureus (MRSA). (Kot et al., 2020) Due to the increased emergence of antibiotic-resistant bacteria, finding new ways to combat these pathogens has become essential. Their ability to form biofilms makes each of these bacteria difficult. Biofilm formation is one of the leading contributors to antibiotic resistance and hospital-related infections. The biofilm allows bacteria to stick and multiply on medical instruments. Biofilms comprise extracellular polymeric substance (EPS), S. Epidermis, and S. Aureus biofilms, which are polysaccharides, amyloids, and pro...
Post a Comment
Read more