Hi Everyone...This week was easy breezy. My morning class was canceled on Wednesday, so I could sleep in for the first time since starting the semester. Regarding TRAIN stuff, I'm working on entering my data in Excel and interpreting it. It might just be me, but working with Excel is exhausting. As a small experiment, I replicated a process I did when I first started TRAIN. I prepared a plate with S.A. and added an antibiotic susceptibility disk dipped in a honey solution. The next time I come in, I'll measure the circle around the disk where no bacteria grows.
On Tuesday, I plated E. Coli and tested the effectiveness of 4 samples to kill the bacteria. The sample included , Aloe Vera gel (store bought), Windex, Tap water, and 70% Isopropyl Alcohol. This experiment was pretty much used to keep me busy as I haven't yet been able to start a project. However when I came in Wednesday, I found some interesting results. The zone of inhibition was basically 0 for every sample, including the isopropyl alcohol. Although the tap water, being the control, and the Windex doesn't jump out as a surprise to me, the 70% Alcohol does. Originally I had two hypothesis's on why this occurred. One being that because E. Coli is a gut bacteria and therefore is built to withstand alcohol. The second theory suggest user error such as the letting the alcohol evaporate to much before I was able to put the disk on the plate. Because of the results on Wednesday and with the first hypothesis in mind, I chose to do another plate instead with Staphylococcus. I ch...
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