(Mueller-Hilton Broth)
Hi everyone! My project this semester is testing Manuka honey as an antimicrobial. It's more complex than my experiment last semester, as I plan on measuring the effectiveness with more processes. Last week I made Mueller-Hilton Broth to grow the bacteria in. I grew multiple samples of Staphylococcus A. this week. Josh and I decided to start with the basics before diving into the complexities of my experiment. I'm working on the best way to dilute and transfer honey into the MH Broth. I used the spectrophotometer to measure the optical density of my samples. The idea is that the optical density will decrease if the honey inhibits the growth.
Hi Everyone, This week was my first whole week in the lab. I focused mainly on calculations and locating everything I needed for the next few weeks. I also made fresh media this week. We made a few adjustments to last year's protocol in hopes that we get better jobs. We made adjustments by ordering flat bottom well plates to help bacterial biofilms stick better. We got an adhesive film to cover the plates to avoid too much evaporation and cross-contamination. By next week, I plan to plate some bacteria and measure the biofilms. (I had to autoclave my media and bottles to store it in)

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