Hi Everyone,
Another week, another post. I can't believe we're almost done with TRAIN, let alone the semester. My research has just started picking up. On Tuesday, I had to hunker down and spend 2+ hours in the lab on one experiment. I'm really excited about my results. For the first time, I was able to definitely prove that the pseudomonas does grow biofilm. However, the biofilm only stuck to the sides because I used plates with u-bottom wells instead of flat-bottom. This is going to make it hard to put the data into numbers. I'm debating whether I want/have time to redo the experiment with flat bottom wells. If not, this might be the end of my research this semester.
On Tuesday, I plated E. Coli and tested the effectiveness of 4 samples to kill the bacteria. The sample included , Aloe Vera gel (store bought), Windex, Tap water, and 70% Isopropyl Alcohol. This experiment was pretty much used to keep me busy as I haven't yet been able to start a project. However when I came in Wednesday, I found some interesting results. The zone of inhibition was basically 0 for every sample, including the isopropyl alcohol. Although the tap water, being the control, and the Windex doesn't jump out as a surprise to me, the 70% Alcohol does. Originally I had two hypothesis's on why this occurred. One being that because E. Coli is a gut bacteria and therefore is built to withstand alcohol. The second theory suggest user error such as the letting the alcohol evaporate to much before I was able to put the disk on the plate. Because of the results on Wednesday and with the first hypothesis in mind, I chose to do another plate instead with Staphylococcus. I ch...
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