Hi Everyone,
This week, I attempted my very first two-fold serial dilution. I was excited about this experiment because it's the next step in determining the effect manuka honey has on Staphylococcus aureus. It's also something I've never done before. It did require a few YouTube videos, but eventually, I understood the concept and what I was supposed to do. Since this is my first time doing this procedure, I consider it a rough draft. My main focus was technique. Ultimately, I noticed some things I'd like to tweak in the future, but it wasn't bad. Next week, I plan on doing another serial dilution with the corrections included.
Hi Everyone, This week was my first whole week in the lab. I focused mainly on calculations and locating everything I needed for the next few weeks. I also made fresh media this week. We made a few adjustments to last year's protocol in hopes that we get better jobs. We made adjustments by ordering flat bottom well plates to help bacterial biofilms stick better. We got an adhesive film to cover the plates to avoid too much evaporation and cross-contamination. By next week, I plan to plate some bacteria and measure the biofilms. (I had to autoclave my media and bottles to store it in)

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