Hi Everyone,
This week, I attempted my very first two-fold serial dilution. I was excited about this experiment because it's the next step in determining the effect manuka honey has on Staphylococcus aureus. It's also something I've never done before. It did require a few YouTube videos, but eventually, I understood the concept and what I was supposed to do. Since this is my first time doing this procedure, I consider it a rough draft. My main focus was technique. Ultimately, I noticed some things I'd like to tweak in the future, but it wasn't bad. Next week, I plan on doing another serial dilution with the corrections included.
On Tuesday, I plated E. Coli and tested the effectiveness of 4 samples to kill the bacteria. The sample included , Aloe Vera gel (store bought), Windex, Tap water, and 70% Isopropyl Alcohol. This experiment was pretty much used to keep me busy as I haven't yet been able to start a project. However when I came in Wednesday, I found some interesting results. The zone of inhibition was basically 0 for every sample, including the isopropyl alcohol. Although the tap water, being the control, and the Windex doesn't jump out as a surprise to me, the 70% Alcohol does. Originally I had two hypothesis's on why this occurred. One being that because E. Coli is a gut bacteria and therefore is built to withstand alcohol. The second theory suggest user error such as the letting the alcohol evaporate to much before I was able to put the disk on the plate. Because of the results on Wednesday and with the first hypothesis in mind, I chose to do another plate instead with Staphylococcus. I ch...
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