Hi everyone,
This week, I started simple. Monday, I took the optical density of my samples(media+SA, HG+SA, HM1+SA, HM2+SA) after 96hrs of incubation. All samples exhibited an increased optical density. These are different from the results we were looking for. I hypothesized that the HM1 and HM2 would inhibit the growth of Staphylococcus Aureus. Had I been correct, the densities would've decreased in HM1+SA and HM2+SA while increasing in Media+SA. However, I'm still optimistic because, despite growth all across the board, HM1+SA and HM2+SA had the smallest increase. The rest of the week, I spent time preparing for another test, hoping to decrease the technical issues I had with the spectrophotometer.
On Tuesday, I plated E. Coli and tested the effectiveness of 4 samples to kill the bacteria. The sample included , Aloe Vera gel (store bought), Windex, Tap water, and 70% Isopropyl Alcohol. This experiment was pretty much used to keep me busy as I haven't yet been able to start a project. However when I came in Wednesday, I found some interesting results. The zone of inhibition was basically 0 for every sample, including the isopropyl alcohol. Although the tap water, being the control, and the Windex doesn't jump out as a surprise to me, the 70% Alcohol does. Originally I had two hypothesis's on why this occurred. One being that because E. Coli is a gut bacteria and therefore is built to withstand alcohol. The second theory suggest user error such as the letting the alcohol evaporate to much before I was able to put the disk on the plate. Because of the results on Wednesday and with the first hypothesis in mind, I chose to do another plate instead with Staphylococcus. I ch...
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